@article {2341,
	title = {New findings for in-gel digestion accelerated by high-intensity focused ultrasound for protein identification by matrix-assisted laser desorption ionization time-of-flight mass spectrometry},
	journal = {Journal of Chromatography A},
	volume = {1153},
	number = {1-2},
	year = {2007},
	note = {<p>Times Cited: 15</p>
},
	month = {Jun 15},
	pages = {291-299},
	abstract = {<p>New findings in sample treatment based on high-intensity focused ultrasound (HIFU) for protein digestion after polyacrylamide gel electrophoresis separation are presented. The following variables were studied: (i) sample volume; (ii) sonotrode diameter; (iii) previous protein denaturation; (iv) cooling; (v) enzyme concentration; and (vi) protein concentration. Results showed that positive protein identification could be done after protein separation by gel electrophoresis through peptide mass fingerprint (PMF) in a volume as low as 25 mu L. The time needed was less than 2 min and no cooling was necessary. The importance of the sonotrode diameter was negligible. On the other hand, protein denaturation before sonication was a trade-off for the success of procedure here described. The protein coverage was raised from 5 to 30\%, and the number of peptides matching the proteins was also increased in a percentage ranging 10-100\% when the classical overnight treatment is compared with the proposed HIFU procedure. The minimum amount of protein that can be identified using the HIFU sample treatment by matrix-assisted laser desorption ionization time-of-flight mass spectrometry (MALDI-TOF-MS) was 0.06 mu g. The lower concentration of trypsin successfully used to obtain an adequate protein digestion was 3.6 mu g/mL. (c) 2006 Elsevier B.V. All rights reserved.</p>
},
	isbn = {0021-9673},
	url = {http://dx.doi.org/10.1016/j.chroma.2006.09.078},
	author = {Carreira, R. J. and Cordeiro, F. M. and Moro, A. J. and Rivas, M. G. and Rial-Otero, R. and Gaspar, E. M. and Moura, I. and Capelo, J. L.}
}
