@article {2337,
	title = {Mediated catalysis of Paracoccus pantotrophus cytochrome c peroxidase by P. pantotrophus pseudoazurin: kinetics of intermolecular electron transfer},
	journal = {J Biol Inorg Chem},
	volume = {12},
	number = {5},
	year = {2007},
	note = {<p>0949-8257 (Print)0949-8257 (Linking)Journal ArticleResearch Support, Non-U.S. Gov{\textquoteright}t</p>
},
	month = {Jun},
	pages = {691-8},
	abstract = {<p>This work reports the direct electrochemistry of Paracoccus pantotrophus pseudoazurin and the mediated catalysis of cytochrome c peroxidase from the same organism. The voltammetric behaviour was examined at a gold membrane electrode, and the studies were performed in the presence of calcium to enable the peroxidase activation. A formal reduction potential, E (0){\textquoteright}, of 230 +/- 5 mV was determined for pseudoazurin at pH 7.0. Its voltammetric signal presented a pH dependence, defined by pK values of 6.5 and 10.5 in the oxidised state and 7.2 in the reduced state, and was constant up to 1 M NaCl. This small copper protein was shown to be competent as an electron donor to cytochrome c peroxidase and the kinetics of intermolecular electron transfer was analysed. A second-order rate constant of 1.4 +/- 0.2 x 10(5) M(-1) s(-1) was determined at 0 M NaCl. This parameter has a maximum at 0.3 M NaCl and is pH-independent between pH 5 and 9.</p>
},
	keywords = {Azurin/*metabolism, Catalysis, Cytochrome-c Peroxidase/*metabolism, Electrochemistry, Electrodes, Electrolytes, Electron Transport/*physiology, Hydrogen Peroxide/chemistry, Hydrogen-Ion Concentration, Indicators and Reagents, Kinetics, Paracoccus pantotrophus/*enzymology},
	url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=PubMed\&dopt=Citation\&list_uids=17361419 },
	author = {de Sousa, P. M. and Pauleta, S. R. and Goncalves, M. L. and Pettigrew, G. W. and Moura, I. and Dos Santos, M. M. and Moura, J. J.}
}
