@article {2307,
	title = {Reduction of ascorbate free radical by the plasma membrane of synaptic terminals from rat brain},
	journal = {Arch Biochem Biophys},
	volume = {469},
	number = {2},
	year = {2008},
	note = {<p>1096-0384 (Electronic)0003-9861 (Linking)Journal ArticleResearch Support, Non-U.S. Gov{\textquoteright}t</p>
},
	month = {Jan 15},
	pages = {243-54},
	abstract = {<p>Synaptic plasma membranes (SPMV) decrease the steady state ascorbate free radical (AFR) concentration of 1mM ascorbate in phosphate/EDTA buffer (pH 7), due to AFR recycling by redox coupling between ascorbate and the ubiquinone content of these membranes. In the presence of NADH, but not NADPH, SPMV catalyse a rapid recycling of AFR which further lower the AFR concentration below 0.05 microM. These results correlate with the nearly 10-fold higher NADH oxidase over NADPH oxidase activity of SPMV. SPMV has NADH-dependent coenzyme Q reductase activity. In the presence of ascorbate the stimulation of the NADH oxidase activity of SPMV by coenzyme Q(1) and cytochrome c can be accounted for by the increase of the AFR concentration generated by the redox pairs ascorbate/coenzyme Q(1) and ascorbate/cytochrome c. The NADH:AFR reductase activity makes a major contribution to the NADH oxidase activity of SPMV and decreases the steady-state AFR concentration well below the micromolar concentration range.</p>
},
	keywords = {*Free Radicals, Animals, Ascorbic Acid/*metabolism, Biochemistry/*methods, Brain/*metabolism, Cell Membrane/*metabolism, Cytochromes c/metabolism, Edetic Acid/chemistry, NADPH Oxidase/metabolism, Oxidation-Reduction, Rats, Synapses/*metabolism, Ubiquinone/chemistry},
	url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=PubMed\&dopt=Citation\&list_uids=17963686 },
	author = {Samhan-Arias, A. K. and Duarte, R. O. and Martin-Romero, F. J. and Moura, J. J. and Gutierrez-Merino, C.}
}
