@article {2266,
	title = {Continuous-wave EPR at 275GHz: application to high-spin Fe(3+) systems},
	journal = {J Magn Reson},
	volume = {210},
	number = {1},
	year = {2011},
	note = {<p>1096-0856 (Electronic)1090-7807 (Linking)Journal ArticleResearch Support, Non-U.S. Gov{\textquoteright}t</p>
},
	month = {May},
	pages = {126-32},
	abstract = {<p>The 275GHz electron-paramagnetic-resonance spectrometer we reported on in 2004 has been equipped with a new probe head, which contains a cavity especially designed for operation in continuous-wave mode. The sensitivity and signal stability that is achieved with this new probe head is illustrated with 275GHz continuous-wave spectra of a 1mM frozen solution of the complex Fe(III)-ethylenediamine tetra-acetic acid and of 10mM frozen solutions of the protein rubredoxin, which contains Fe(3+) in its active site, from three different organisms. The high quality of the spectra of the rubredoxins allows the determination of the zero-field-splitting parameters with an accuracy of 0.5GHz. The success of our approach results partially from the enhanced absolute sensitivity, which can be reached using a single-mode cavity. At least as important is the signal stability that we were able to achieve with the new probe head.</p>
},
	keywords = {Anisotropy, Edetic Acid/chemistry, Electron Spin Resonance Spectroscopy/instrumentation/*methods, Equipment Design, Ferrous Compounds/*chemistry, Rubredoxins/*chemistry, Sensitivity and Specificity, Solutions/chemistry, Spin Labels},
	url = {http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve\&db=PubMed\&dopt=Citation\&list_uids=21444227 },
	author = {Mathies, G. and Blok, H. and Disselhorst, J. A. and Gast, P. and van der Meer, H. and Miedema, D. M. and Almeida, R. M. and Moura, J. J. and Hagen, W. R. and Groenen, E. J.}
}
