@article {8484, title = {Unravelling glucan recognition systems by glycome microarrays using the designer approach and mass spectrometry}, journal = {Mol Cell Proteomics}, year = {2015}, note = {n/a}, type = {Journal Article}, abstract = {

Glucans are polymers of D-glucose with differing linkages in linear or branched sequences. They are constituents of microbial and plant cell-walls and involved in important bio-recognition processes including immunomodulation, anti-cancer activities, pathogen virulence and plant cell-wall biodegradation. Translational possibilities for these activities in medicine and biotechnology are considerable. High-throughput micro-methods are needed to screen proteins for recognition of specific glucan sequences as a lead to structure-function studies and their exploitation. We describe construction of a glucome microarray, the first sequence-defined glycome-scale microarray, using a designer approach from targeted ligand-bearing glucans in conjunction with a novel high-sensitivity mass spectrometric sequencing method, as a screening tool to assign glucan recognition motifs. The glucome microarray comprises 153 oligosaccharide probes with high purity, representing major sequences in glucans. The negative-ion electrospray tandem mass spectrometry with collision-induced dissociation was used for complete linkage analysis of gluco-oligosaccharides in linear homo and hetero and branched sequences. The system is validated using antibodies and carbohydrate-binding modules known to target α- or β-glucans in different biological contexts, extending knowledge on their specificities, and applied to reveal new information on glucan recognition by two signalling molecules of the immune system against pathogens: Dectin-1 and DC-SIGN. The sequencing of the glucan oligosaccharides by the MS method and their interrogation on the microarrays provides detailed information on linkage, sequence and chain length requirements of glucan-recognizing proteins, and are a sensitive means of revealing unsuspected sequences in the polysaccharides.

}, issn = {1535-9484}, doi = {10.1074/mcp.M115.048272}, url = {http://www.ncbi.nlm.nih.gov/pubmed/25670804}, author = {Palma, A. S. and Liu, Y. and Zhang, H. and Zhang, Y. and McCleary, B. V. and Yu, G. and Huang, Q. and Guidolin, L. S. and Ciocchini, A. E. and Torosantucci, A. and Wang, D. and Carvalho, A. L. and Fontes, C. M. and Mulloy, B. and Childs, R. A. and Feizi, T. and Chai, W.} }